Difference between revisions of "Part:BBa K2348012"
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Revision as of 15:55, 1 November 2017
alx promoter and riboswitch + mNeonGreen
This part actually is a composite part containing the alx promoter and riboswitch BBa_K2348000 and mNeonGreen BBa_K1761003. But in addition, between riboswitch and mNeonGreen a TEV-site and a f-degron are inserted. Also, mNeonGreen ins 6xHis-taged and the parts ends with a T7-terminator. Because the part does not work when build as Biobrick due to the Biobrick scar this part is listed as basic part.
TEV-site and f-degron allow a fast and controllable degradation of mNeonGreen and in combination with the fast maturation time of mNeonGreen allow a fast cooler change when used together with our other pH-sensitive part BBa_K2348011
Sequence and Features
<a href="
"><img src="Alx_part_Graz.jpeg/799px-Alx_part_Graz.jpegg" alt="Bild" /></a>
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 312