Difference between revisions of "Part:BBa K2524002"

Revision as of 15:54, 1 November 2017

Limulus Clotting Factor C-hCG fusion protein

The Factor C-hCG fusion protein can allow for the detection of gram-negative bacteria in a solution. With hCG fused to the end of Factor C that is immobilized in a solution, it can be used as a detection signal for the activation of Factor C. When LPS is introduced into the solution the hCG will be cut and be released into the solution. When a pregnancy test is inserted the pregnancy test will produce a positive result. The hCG is fused to the end of the Light chain of the Factor C.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2524002 short</partinfo>
-Factor C is an enzyme produced in the Horseshoe crab. It is apart of their immune system. The Factor C protein is the first in a cascade that causes their blood to clot in the presence of lipopolysaccharide (LPS) located on the outer membrane of gram-negative bacteria. When Factor C is in the presence of LPS it undergoes autocatalysis. This is very helpful upon detection of minuscule concentrations of gram-negative bacteria.
+ The Factor C-hCG fusion protein can allow for the detection of gram-negative bacteria in a solution. With hCG fused to the end of Factor C that is immobilized in a solution, it can be used as a detection signal for the activation of Factor C. When LPS is introduced into the solution the hCG will be cut and be released into the solution. When a pregnancy test is inserted the pregnancy test will produce a positive result. The hCG is fused to the end of the Light chain of the Factor C.
+<!-- Factor C is an enzyme produced in the Horseshoe crab. It is apart of their immune system. The Factor C protein is the first in a cascade that causes their blood to clot in the presence of lipopolysaccharide (LPS) located on the outer membrane of gram-negative bacteria. When Factor C is in the presence of LPS it undergoes autocatalysis. This is very helpful upon detection of minuscule concentrations of gram-negative bacteria. Factor C is a glycoprotein with a molecular mass of 123 kD. It has an Heavy chain (80kD) and Light chain (43 kD). In the presence of LPS, factor C is activated and undergoes autocatalysis and cleaves the Phe-Ile bond. This autocatalysis results in the formation of two new fragments: B chain (34kD) and A chain (8.5 kD).
+Human Chorionic Gonadotropin (hCG)  is the hormone produced in women when they become pregnant. The hCG Beta-subunit has the epitopes that are detected by pregnancy tests. When hCG is present in a womans pee a pregnancy test will produce a positive result.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===