Difference between revisions of "Part:BBa K2381007"

 
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<partinfo>BBa_K2381007 short</partinfo>
 
<partinfo>BBa_K2381007 short</partinfo>
  
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the split dCas9 is expressed alone.
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It is the other part of rational designed split dCas9. The design is from split-Cas9 enzyme in which Cas9 is split into two lobes according to its function. This part of dCas9 is a α-helical lobe corresponding to Cas9.  
  
 
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Revision as of 15:43, 1 November 2017


Rational split dCas9-2

It is the other part of rational designed split dCas9. The design is from split-Cas9 enzyme in which Cas9 is split into two lobes according to its function. This part of dCas9 is a α-helical lobe corresponding to Cas9.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 934
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]