Difference between revisions of "Part:BBa K2379010:Design"

(→‎Design Notes)
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===References===
 
===References===
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1. Konan, K.V. & Yanofsky, C. Rho-Dependent transcription termination in the tna Operon of Escherichia coli: Roles of the boxA Sequence and the rut Site.J. Bacteriol. 182(14), 3981–3988 (2000).
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2. Chang, C. L.,Lei Qi, Lucks J.B., Segall-Shapiro T.H., Wang, D.,Mutalik V.K.&  Arkin, A.P.An adaptor from translational to transcriptional control enables predictable assembly of complex regulation. Nat. Methods 9,1088–1094(2012).
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3. Richardson J.P. Loading Rho to terminate transcription. Cell 114, 157–159 (2003).

Revision as of 15:28, 1 November 2017


Adaptor with FourU RNA thermometer


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1068


Design Notes

The circuit is under the regulation of the pLAC promoter. When inserted in a high copy number plasmid pLAC behaves as a constitutive promoter and hence does not require IPTG induction. However, if inserted in a low copy number plasmid, the system will have to be induced with IPTG as it would then behave like an inducible promoter. GFP, in this case, will be produced only in the presence of IPTG and at 37 degree Celsius.

Source

iGEM Registry

References

1. Konan, K.V. & Yanofsky, C. Rho-Dependent transcription termination in the tna Operon of Escherichia coli: Roles of the boxA Sequence and the rut Site.J. Bacteriol. 182(14), 3981–3988 (2000). 2. Chang, C. L.,Lei Qi, Lucks J.B., Segall-Shapiro T.H., Wang, D.,Mutalik V.K.& Arkin, A.P.An adaptor from translational to transcriptional control enables predictable assembly of complex regulation. Nat. Methods 9,1088–1094(2012). 3. Richardson J.P. Loading Rho to terminate transcription. Cell 114, 157–159 (2003).