Difference between revisions of "Part:BBa K2381006:Design"

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===References===
 
===References===
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1. Wright A V, Sternberg S H, Taylor D W, et al. Rational design of a split-Cas9 enzyme complex[J]. Proceedings of the National Academy of Sciences of the United States of America, 2015, 112(10): 2984-2989.
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Wright, Addison V., et al. "Rational design of a split-Cas9 enzyme complex." Proceedings of the National Academy of Sciences of the United States of America 112.10 (2015): 2984-2989.

Revision as of 15:27, 1 November 2017


Rational split dCas9-1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1374
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

the split method is cutting the dCas9 at the end of amino acid 57 and the start of amino acid 729. Then we connect them with a GSS linker.

Source

The dCas9 is from Streptococcus pyogenes.

References

Wright, Addison V., et al. "Rational design of a split-Cas9 enzyme complex." Proceedings of the National Academy of Sciences of the United States of America 112.10 (2015): 2984-2989.