Difference between revisions of "Part:BBa K2381006:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | the split method is cutting at the end of amino acid 57 and the start of amino acid 729. Then | + | the split method is cutting the dCas9 at the end of amino acid 57 and the start of amino acid 729. Then we connect them with a GSS linker. |
| − | + | ||
| − | |||
| − | |||
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===Source=== | ===Source=== | ||
| − | + | Streptococcus pyogenes | |
| − | + | ||
===References=== | ===References=== | ||
| + | 1. Wright A V, Sternberg S H, Taylor D W, et al. Rational design of a split-Cas9 enzyme complex[J]. Proceedings of the National Academy of Sciences of the United States of America, 2015, 112(10): 2984-2989. | ||
Revision as of 15:09, 1 November 2017
Rational split dCas9-1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1374
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
the split method is cutting the dCas9 at the end of amino acid 57 and the start of amino acid 729. Then we connect them with a GSS linker.
Source
Streptococcus pyogenes
References
1. Wright A V, Sternberg S H, Taylor D W, et al. Rational design of a split-Cas9 enzyme complex[J]. Proceedings of the National Academy of Sciences of the United States of America, 2015, 112(10): 2984-2989.