Difference between revisions of "Part:BBa K2381006:Design"

 
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===Design Notes===
 
===Design Notes===
−
the split method is cut it at the loop
+
the split method is cutting at the end of amino acid 57 and the start of amino acid 729. Then connectting them with GSS linker
 +
 
 +
  
  

Revision as of 14:58, 1 November 2017


Rational split dCas9-1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1374
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

the split method is cutting at the end of amino acid 57 and the start of amino acid 729. Then connectting them with GSS linker



Source

no

References