Difference between revisions of "Part:BBa K2379001:Design"

(→‎Design Notes)
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===References===
 
===References===
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1. Konan, K.V. & Yanofsky, C. Rho-Dependent transcription termination in the tna Operon of Escherichia coli: Roles of the boxA Sequence and the rut Site.J. Bacteriol. 182(14), 3981–3988 (2000).
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2. Richardson J.P. Loading Rho to terminate transcription. Cell 114, 157–159 (2003).

Revision as of 14:57, 1 November 2017


This part contains a rho dependent terminator region followed by a ribosome binding site.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part already has a constitutive RBS attached to its end. Hence any team intending to use this part can just add a scar site and the coding sequence downstream of this part.

Source

[ Escherichia coli str. K-12 substr. MG1655 ] Genbank

References

1. Konan, K.V. & Yanofsky, C. Rho-Dependent transcription termination in the tna Operon of Escherichia coli: Roles of the boxA Sequence and the rut Site.J. Bacteriol. 182(14), 3981–3988 (2000).

2. Richardson J.P. Loading Rho to terminate transcription. Cell 114, 157–159 (2003).