Difference between revisions of "Part:BBa K2398529:Design"

 
 
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<partinfo>BBa_K2398529 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2398529 SequenceAndFeatures</partinfo>
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 +
This part contains a fully functional beta-lactamase expression cassette. E. coli beta-lactamase is under control of the constitutive wildtype E. coli promoter. Expression is terminated by the his-operon terminator of pSB1C3. <br><br>
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In this part and related parts, mutations are introduced into the coding sequence. These mutations modify the enzyme activity (see results).
  
  
 
===Design Notes===
 
===Design Notes===
−
todo
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compatible with RFC10
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===Source===
 
===Source===
  
−
todo
+
genomic with modification
  
 
===References===
 
===References===

Latest revision as of 14:54, 1 November 2017


beta-lactamase_Y103D_M67G_F70G


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

This part contains a fully functional beta-lactamase expression cassette. E. coli beta-lactamase is under control of the constitutive wildtype E. coli promoter. Expression is terminated by the his-operon terminator of pSB1C3.

In this part and related parts, mutations are introduced into the coding sequence. These mutations modify the enzyme activity (see results).


Design Notes

compatible with RFC10



Source

genomic with modification

References