Difference between revisions of "Part:BBa K2477013"
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This part constitutively expresses the CueR repressor. This repressor then inhibits the expression of RFP from the downstream PcopA promoter. In the presence of copper, the repression of CueR is inhibited leading to greater RFP expression. Fluorescence of this construct has been shown to increase with increasing copper concentration in both a qualitative and quantitative manner. | This part constitutively expresses the CueR repressor. This repressor then inhibits the expression of RFP from the downstream PcopA promoter. In the presence of copper, the repression of CueR is inhibited leading to greater RFP expression. Fluorescence of this construct has been shown to increase with increasing copper concentration in both a qualitative and quantitative manner. | ||
| − | [[File:UMarylandcoppercircuit.png]] | + | [[File:UMarylandcoppercircuit.png|900px|thumb|center|Copper detection circuit diagram |
| + | A: Copper biosensor in the presence of copper ions | ||
| + | Copper ions inhibit the binding of CueR to the pCopA promoter, allowing for expression of RFP. | ||
| + | |||
| + | B: Copper biosensor in the absence of copper ions | ||
| + | In the absence of copper ions, CueR binds to the pCopA promoter and inhibits RFP expression. A reduced amount of RFP is synthesized due to some leaky expression of the RFP gene. | ||
| + | ]] | ||
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Revision as of 14:50, 1 November 2017
Copper Biosensor
This part constitutively expresses the CueR repressor. This repressor then inhibits the expression of RFP from the downstream PcopA promoter. In the presence of copper, the repression of CueR is inhibited leading to greater RFP expression. Fluorescence of this construct has been shown to increase with increasing copper concentration in both a qualitative and quantitative manner.
Copper detection circuit diagram A: Copper biosensor in the presence of copper ions Copper ions inhibit the binding of CueR to the pCopA promoter, allowing for expression of RFP. B: Copper biosensor in the absence of copper ions In the absence of copper ions, CueR binds to the pCopA promoter and inhibits RFP expression. A reduced amount of RFP is synthesized due to some leaky expression of the RFP gene.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1237
Illegal AgeI site found at 1349 - 1000COMPATIBLE WITH RFC[1000]