Difference between revisions of "Part:BBa K2398527:Design"

 
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<partinfo>BBa_K2398527 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2398527 SequenceAndFeatures</partinfo>
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This part contains a fully functional beta-lactamase expression cassette. E. coli beta-lactamase is under control of the constitutive wildtype E. coli promoter. Expression is terminated by the his-operon terminator of pSB1C3. <br><br>
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In this part and related parts, mutations are introduced into the coding sequence. These mutations modify the enzyme activity (see results).
  
  
 
===Design Notes===
 
===Design Notes===
−
todo
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compatible with RFC10
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===Source===
 
===Source===
  
−
todo
+
genomic with modification
  
 
===References===
 
===References===

Revision as of 14:05, 1 November 2017


beta-lactamase_D129L_L218D


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 651
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


This part contains a fully functional beta-lactamase expression cassette. E. coli beta-lactamase is under control of the constitutive wildtype E. coli promoter. Expression is terminated by the his-operon terminator of pSB1C3.

In this part and related parts, mutations are introduced into the coding sequence. These mutations modify the enzyme activity (see results).


Design Notes

compatible with RFC10



Source

genomic with modification

References