Difference between revisions of "Part:BBa K2505031:Design"

 
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===Design Notes===
 
===Design Notes===
−
None.
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The gene <i>traI</i>(K34G) is derived from <i>Agrobacterium tumefaciens</i> and encode a enzyme necessary for synthesizing Quorum Sensing signaling molecules ([N-]acyl-homoserine lactones, AHLs), 3OC8 HSL (hereafter C8), in <i>E. coli</i>.
 +
This part constitutively produces C8.
 +
 
 +
The mutation was introduced wild type <i>traI</i>(<partinfo>BBa_K553001</partinfo>).
 +
 
 +
The DNA sequences of <i>traI</i> (K34G) is optimized for expressing in <i>E. coli</i> considering the codon usage.
  
  
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===Source===
 
===Source===
  
−
non
+
Mutation was newly introduced on the <partinfo>BBa_K553001</partinfo> by PCR.
  
 
===References===
 
===References===
 +
[1] Pavan Kumar Reddy Kambam, Daniel J. Sayut, Yan Niu, Dawn T. Eriksen, Lianhong Sun (2008) Directed evolution of LuxI for enhanced OHHL production. Biotechnology and Bioengineering Volume 101, Issue 2 1 October 2008 Pages 263-272<br>
 +
 +
[2] MATTHEW R. PARSEK, DALE L. VAL, BRIAN L. HANZELKA, JOHN E. CRONAN, E. P. GREENBERG (1999) Acyl homoserine-lactone quorum-sensing signal generation. Proc. Natl. Acad. Sci. USA Vol. 96, pp. 4360-4365, April 1999 Biochemistry<br>

Revision as of 13:43, 1 November 2017


Ptet-rbs-traI(Q63G)-tt


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The gene traI(K34G) is derived from Agrobacterium tumefaciens and encode a enzyme necessary for synthesizing Quorum Sensing signaling molecules ([N-]acyl-homoserine lactones, AHLs), 3OC8 HSL (hereafter C8), in E. coli. This part constitutively produces C8.

The mutation was introduced wild type traI(BBa_K553001).

The DNA sequences of traI (K34G) is optimized for expressing in E. coli considering the codon usage.


Source

Mutation was newly introduced on the BBa_K553001 by PCR.

References

[1] Pavan Kumar Reddy Kambam, Daniel J. Sayut, Yan Niu, Dawn T. Eriksen, Lianhong Sun (2008) Directed evolution of LuxI for enhanced OHHL production. Biotechnology and Bioengineering Volume 101, Issue 2 1 October 2008 Pages 263-272

[2] MATTHEW R. PARSEK, DALE L. VAL, BRIAN L. HANZELKA, JOHN E. CRONAN, E. P. GREENBERG (1999) Acyl homoserine-lactone quorum-sensing signal generation. Proc. Natl. Acad. Sci. USA Vol. 96, pp. 4360-4365, April 1999 Biochemistry