Difference between revisions of "Part:BBa K2384004"
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DsbA, a protein that can export its C-terminal fusion protein into periplasm more efficiently and faster than maltose binding protein, was used as the key component to express our Metal Binding Peptide periplasmically . | DsbA, a protein that can export its C-terminal fusion protein into periplasm more efficiently and faster than maltose binding protein, was used as the key component to express our Metal Binding Peptide periplasmically . | ||
| + | Our sequence optimization is based on the sequence provided by Peking University's IGEM VIKI in 2010.It is more suitable for expression in Bacillus | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 13:31, 1 November 2017
DsbA+MBP(Hg)
DsbA, a protein that can export its C-terminal fusion protein into periplasm more efficiently and faster than maltose binding protein, was used as the key component to express our Metal Binding Peptide periplasmically .
Our sequence optimization is based on the sequence provided by Peking University's IGEM VIKI in 2010.It is more suitable for expression in Bacillus Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 255
Illegal BglII site found at 267
Illegal BglII site found at 711 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]