Difference between revisions of "Part:BBa K2381001"
Line 3: | Line 3: | ||
<partinfo>BBa_K2381001 short</partinfo> | <partinfo>BBa_K2381001 short</partinfo> | ||
− | nMag, a light-induced dimerization protein derived from VVD protein family, mutates at I52D/M55G. Its corresponding protein, nMag also derived from the VVD protein, mutates at I52R/M55R. They can heterodimerize under blue light irritation (470nm, 3mW/cm2). Their light sensing molecule is flavin adenine dinucleotide (FAD) which is normal in nearly all organisms. <br/> | + | <p>nMag, a light-induced dimerization protein derived from VVD protein family, mutates at I52D/M55G. Its corresponding protein, nMag also derived from the VVD protein, mutates at I52R/M55R. They can heterodimerize under blue light irritation (470nm, 3mW/cm2). Their light sensing molecule is flavin adenine dinucleotide (FAD) which is normal in nearly all organisms. <br/> |
− | This part is nMag-High, whose binding efficiency is much higher than the origin nMag. It's function is proved by parts | + | This part is nMag-High, whose binding efficiency is much higher than the origin nMag. It's function is proved by parts <partinfo>BBa_K2381019.</partinfo> <br/> |
If you want to use it, remember to fuse this part with the part you are interested in through a 12aa-linker. | If you want to use it, remember to fuse this part with the part you are interested in through a 12aa-linker. | ||
− | + | </p> | |
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== |
Revision as of 13:27, 1 November 2017
nMagHigh
nMag, a light-induced dimerization protein derived from VVD protein family, mutates at I52D/M55G. Its corresponding protein, nMag also derived from the VVD protein, mutates at I52R/M55R. They can heterodimerize under blue light irritation (470nm, 3mW/cm2). Their light sensing molecule is flavin adenine dinucleotide (FAD) which is normal in nearly all organisms.
This part is nMag-High, whose binding efficiency is much higher than the origin nMag. It's function is proved by parts No part name specified with partinfo tag.
If you want to use it, remember to fuse this part with the part you are interested in through a 12aa-linker.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 403