Difference between revisions of "Part:BBa K2507000"
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ThsS/R are two basic parts which belong to the two-component systems. This system is from a salmonella species. After validate this system in laboratory Escherichia coli, this system can function as a thiosulfate sensor and reporter. Because of thiosulfate is an indicator of gut inflammation, this system can be used as a sensor of gut inflammation. Connect thsR with sfGFP or chromoprotein， this system can response to thiosulfate by produce protein.
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ThsS (BBa_K2507000) and ThsR (BBa_K2507001), both codon-optimized for E. coli, are two basic parts which belong to the two-component system from the marine bacterium Shewanella halifaxensis. ThsS is the membrane-bound sensor kinase (SK) which can sense thiosulfate outside the cell, and ThsR is the DNA-binding response regulator(RR).
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Revision as of 13:26, 1 November 2017

ThsS

Background

ThsS (BBa_K2507000) and ThsR (BBa_K2507001), both codon-optimized for E. coli, are two basic parts which belong to the two-component system from the marine bacterium Shewanella halifaxensis. ThsS is the membrane-bound sensor kinase (SK) which can sense thiosulfate outside the cell, and ThsR is the DNA-binding response regulator(RR).



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1078
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 477
    Illegal BamHI site found at 525
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1010



Results