Difference between revisions of "Part:BBa K2201026"
| Line 5: | Line 5: | ||
Plasmid for the retention of unnatural base pairs | Plasmid for the retention of unnatural base pairs | ||
| − | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | <html> | ||
| + | This part was used as a backbone for the integration of fragments containing unnatural base pairs. The insertion site is located upstream of mRFP, providing an easy screening method. For more on the retention and preservation system, please visit our <a href="http://2017.igem.org/Team:Bielefeld-CeBiTec/Project/unnatural_base_pair/preservation_system">wiki!</a> | ||
| + | </html> | ||
| − | |||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K2201026 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2201026 SequenceAndFeatures</partinfo> | ||
Revision as of 13:23, 1 November 2017
Plasmid for the integration of unnatural base pairs
Plasmid for the retention of unnatural base pairs
Usage and Biology
This part was used as a backbone for the integration of fragments containing unnatural base pairs. The insertion site is located upstream of mRFP, providing an easy screening method. For more on the retention and preservation system, please visit our wiki!
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 616
Illegal AgeI site found at 728 - 1000COMPATIBLE WITH RFC[1000]