Difference between revisions of "Part:BBa K2398503:Design"
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<partinfo>BBa_K2398503 short</partinfo>
 
<partinfo>BBa_K2398503 short</partinfo>
  
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Design:
 
 
This part contains a fully functional beta-lactamase expression cassette. E. coli beta-lactamase is under control of the constitutive wildtype E. coli promoter. Expression is terminated by the his-operon terminator of pSB1C3. <br><br>
 
This part contains a fully functional beta-lactamase expression cassette. E. coli beta-lactamase is under control of the constitutive wildtype E. coli promoter. Expression is terminated by the his-operon terminator of pSB1C3. <br><br>
 
In this part and related parts, mutations are introduced into the coding sequence. These mutations modify the enzyme activity (see results).
 
In this part and related parts, mutations are introduced into the coding sequence. These mutations modify the enzyme activity (see results).

Revision as of 13:16, 1 November 2017


beta-lactamase_ Y103D_M67G_F70G

This part contains a fully functional beta-lactamase expression cassette. E. coli beta-lactamase is under control of the constitutive wildtype E. coli promoter. Expression is terminated by the his-operon terminator of pSB1C3.

In this part and related parts, mutations are introduced into the coding sequence. These mutations modify the enzyme activity (see results).



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

compatible with RFC10 and Goldengate


Source

genomic, with modifications

References