Difference between revisions of "Part:BBa K2201010"
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__NOTOC__
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<partinfo>BBa_K2201010 short</partinfo>
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Construct comprised of PtNTT2, lacO and Cas9
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===Usage and Biology===
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<html>
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This part was used as a part of the retention and preservation system for unnatural base pairs. For a comprehensive characterization of <i>Pt</i>NTT2,
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please view <a href="https://parts.igem.org/Part:BBa_K2201004">BBa_K2201004</a>. A characterization of the lacO operon and Cas9 can be found here: <a href="https://parts.igem.org/Part:BBa_K2201020">BBa_K2201020</a> and <a href="https://parts.igem.org/Part:BBa_K2201023">BBa_K2201023</a>.
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</html>
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2201010 SequenceAndFeatures</partinfo>
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<!-- Uncomment this to enable Functional Parameter display
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===Functional Parameters===
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<partinfo>BBa_K2201010 parameters</partinfo>
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<!-- -->
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2201010 short</partinfo>
 
<partinfo>BBa_K2201010 short</partinfo>

Revision as of 13:12, 1 November 2017


Construct comprised of PtNTT2, lacO and Cas9

Construct comprised of PtNTT2, lacO and Cas9

Usage and Biology

This part was used as a part of the retention and preservation system for unnatural base pairs. For a comprehensive characterization of PtNTT2, please view BBa_K2201004. A characterization of the lacO operon and Cas9 can be found here: BBa_K2201020 and BBa_K2201023.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3356
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3356
    Illegal NheI site found at 3115
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3356
    Illegal BamHI site found at 5394
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3356
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3356
  • 1000
    COMPATIBLE WITH RFC[1000]


Construct comprised of PtNTT2, lacO and Cas9

Construct comprised of PtNTT2, lacO and Cas9

Usage and Biology

This part was used as a part of the retention and preservation system for unnatural base pairs. For a comprehensive characterization of PtNTT2, please view <a href="https://parts.igem.org/Part:BBa_K2201004"> BBa_K2201004</a>. A characterization of the lacO operon and Cas9 can be found here: <a href="https://parts.igem.org/Part:BBa_K2201020">BBa_K2201020</a> and <a href="https://parts.igem.org/Part:BBa_K2201023">BBa_K2201023</a>. Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3356
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3356
    Illegal NheI site found at 3115
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3356
    Illegal BamHI site found at 5394
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3356
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3356
  • 1000
    COMPATIBLE WITH RFC[1000]