Difference between revisions of "Part:BBa K2201010"

Revision as of 13:10, 1 November 2017

Construct comprised of PtNTT2, lacO and Cas9

This part was used as a part of the retention and preservation system for unnatural base pairs. For a comprehensive characterization of PtNTT2, please view <a href="https://parts.igem.org/Part:BBa_K2201004"> BBa_K2201004</a>. A characterization of the lacO operon and Cas9 can be found here: <a href="https://parts.igem.org/Part:BBa_K2201020">BBa_K2201020</a> and <a href="https://parts.igem.org/Part:BBa_K2201023">BBa_K2201023</a>. Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 3356
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 3356
Illegal NheI site found at 3115
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 3356
Illegal BamHI site found at 5394
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 3356
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 3356
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K2201010 short</partinfo>
@@ Line 5: / Line 4: @@
 Construct comprised of PtNTT2, lacO and Cas9
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
-<!-- -->
+This part was used as a part of the retention and preservation system for unnatural base pairs. For a comprehensive characterization of <i>Pt</i>NTT2,
+please view <a href="https://parts.igem.org/Part:BBa_K2201004"> BBa_K2201004</a>. A characterization of the lacO operon and Cas9 can be found here: <a href="https://parts.igem.org/Part:BBa_K2201020">BBa_K2201020</a> and <a href="https://parts.igem.org/Part:BBa_K2201023">BBa_K2201023</a>.
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K2201010 SequenceAndFeatures</partinfo>