Difference between revisions of "Part:BBa K2309032"

 
 
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<partinfo>BBa_K2309032 short</partinfo>
 
<partinfo>BBa_K2309032 short</partinfo>
  
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This primer contain 17 bp pf BioBrick suffix and the rest for Gibson Assembly. PstI is used to linearize pNZ8148.
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This primer contain 17 bp of the standard BioBrick suffix and the rest can be digested and ligated into pNZ8148 at the restriciton site PstI.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 12:25, 1 November 2017


Standard reverse primer for Gibson Assembly to pNZ8148

This primer contain 17 bp of the standard BioBrick suffix and the rest can be digested and ligated into pNZ8148 at the restriciton site PstI.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 22
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 22
    Illegal NotI site found at 27
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 22
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 22
  • 1000
    COMPATIBLE WITH RFC[1000]