Difference between revisions of "Part:BBa K2446030:Design"
| Line 12: | Line 12: | ||
===Source=== | ===Source=== | ||
| − | his part is assembled by Sv40 promoter BBa_K2446052 and 4*PIP DNA binding sites | + | his part is assembled by Sv40 promoter BBa_K2446052 and 4*PIP DNA binding sites BBa_K2446015. |
===References=== | ===References=== | ||
Revision as of 11:54, 1 November 2017
SV40_8_ZF_21-16 promoter
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is aseembled by the specfic DNA binding sites and Sv40 promoter through Biobrick assembly. We inset the DNA binding sites behind the Sv40 promoter. Then the DNA binding domain with KRAB can repress the expression of this promoter. The repression intensity can be adjusted by change the repeats of DNA binding sites.
Source
his part is assembled by Sv40 promoter BBa_K2446052 and 4*PIP DNA binding sites BBa_K2446015.
References
[1] J. J. Lohmueller, T. Z. Armel, P. A. Silver, A tunable zinc finger-based framework for Boolean logic computation in mammalian cells. Nucleic Acids Research 40, 5180--5187 (2012).
[2] R. Witzgall, E. O'Leary, A. Leaf, D. Onaldi, J. V. Bonventre, The Krüppel-associated box-A (KRAB-A) domain of zinc finger proteins mediates transcriptional repression. Proceedings of the National Academy of Sciences 91, 4514-4518 (1994).