Difference between revisions of "Part:BBa K1979001"

Revision as of 11:48, 1 November 2017

CBD (cbh2)

The sequence codes for cellulose binding domain cbh2 in prokaryotic cells.

Proof of the CBD function

The resulted solution after expression is mixed with a gauze piece, and the green color on gauze is recorded. Then the gauze is washed three times using ddH2O, but the green fluorescent on it is not significantly reduced, proving that the CDB is well functioned. In comparison, no green fluorescent is left after washing the gauze mixed with GFP-ChBD (Chintin binding domain).

[Fig. 2. GFP-CBD on gauze before washing]

[Fig. 3. GFP-CBD on gauze after washing]

[Fig. 4. GFP-ChBD on gauze before washing]

[Fig. 5. GFP-ChBD on gauze after washing]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 11

@@ Line 5: / Line 5: @@
 The sequence codes for cellulose binding domain cbh2 in prokaryotic cells.
-<!-- Add more about the biology of this part here
+<h4>Proof of the CBD function</h4>
-===Usage and Biology===
+The resulted solution after expression is mixed with a gauze piece, and the green color on gauze is recorded. Then the gauze is washed three times using ddH2O, but the green fluorescent on it is not significantly reduced, proving that the CDB is well functioned. In comparison, no green fluorescent is left after washing the gauze mixed with GFP-ChBD (Chintin binding domain).
+[[file:cbd-before.jpeg|150px]]
+[Fig. 2. GFP-CBD on gauze before washing]
+[[file:cbd-after.jpeg|150px]]
+[Fig. 3. GFP-CBD on gauze after washing]
+[[file:chbd-before.jpeg|150px]]
+[Fig. 4. GFP-ChBD on gauze before washing]
+[[file:chbd-after.jpeg|150px]]
+[Fig. 5. GFP-ChBD on gauze after washing]
 <!-- -->