Difference between revisions of "Part:BBa K2483002"
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<partinfo>BBa_K2483002 short</partinfo> | <partinfo>BBa_K2483002 short</partinfo> | ||
| − | This part is designed to produce dCas9 when Lac or IPTG is present. LacI-LVA (BBa_C0012) is constitutively expressed under the pRep4 promoter and dCas9 has the Lac promoter (BBa_R0010) at the 5'-end. | + | This part is designed to produce dCas9 when Lac or IPTG is present. LacI-LVA (BBa_C0012: https://parts.igem.org/Part:BBa_C0012) is constitutively expressed under the pRep4 promoter and dCas9 has the Lac promoter (BBa_R0010: https://parts.igem.org/Part:BBa_R0010) at the 5'-end. |
| − | This part was used in our metabolic channelling project and is also part of BBa_K2483004, our final construct for our experiments. | + | This part was used in our metabolic channelling project and is also part of BBa_K2483004: https://parts.igem.org/Part:BBa_K2483004, our final construct for our experiments. |
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Latest revision as of 11:24, 1 November 2017
Lac regulated dCas9 with LacI constitutively expressed
This part is designed to produce dCas9 when Lac or IPTG is present. LacI-LVA (BBa_C0012: https://parts.igem.org/Part:BBa_C0012) is constitutively expressed under the pRep4 promoter and dCas9 has the Lac promoter (BBa_R0010: https://parts.igem.org/Part:BBa_R0010) at the 5'-end.
This part was used in our metabolic channelling project and is also part of BBa_K2483004: https://parts.igem.org/Part:BBa_K2483004, our final construct for our experiments.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 2666
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1258
Illegal BamHI site found at 4945 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]