Difference between revisions of "Part:BBa K2398503:Design"

 
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__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K2398503 short</partinfo>
 
<partinfo>BBa_K2398503 short</partinfo>
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Design:
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This part contains a fully functional beta-lactamase expression cassette. E. coli beta-lactamase is under control of the constitutive wildtype E. coli promoter. Expression is terminated by the his-operon terminator of pSB1C3. <br><br>
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In this part and related parts, mutations are introduced into the coding sequence. These mutations modify the enzyme activity (see results).
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<partinfo>BBa_K2398503 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2398503 SequenceAndFeatures</partinfo>

Revision as of 11:19, 1 November 2017


beta-lactamase_ Y103D_M67G_F70G

Design: This part contains a fully functional beta-lactamase expression cassette. E. coli beta-lactamase is under control of the constitutive wildtype E. coli promoter. Expression is terminated by the his-operon terminator of pSB1C3.

In this part and related parts, mutations are introduced into the coding sequence. These mutations modify the enzyme activity (see results).



Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Unknown
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

compatible with RFC10 and Goldengate


Source

genomic, with modifications

References