Difference between revisions of "Part:BBa K2448033"

Revision as of 11:09, 1 November 2017

D-Psicose 3-epimerase (Dpe) from Clostridium cellulolyticum under the control of pTacI promoter

D-Psicose 3-epimerase (Dpe) from Clostridium cellulolyticum

This part is an E. coli codon optimized version of the D-Psicose 3-epimerase (Dpe) from Clostridium cellulolyticum str. ATCC 35319 (Uniprot B8I944, BBa_K2448021).equipped with a strong RBS (BBa_K2448019) and placed under the control of the pTacI promoter (BBa_K864400).

Usage and Biology

D-Psicose 3-epimerase (EC: 5.1.3.30) catalyses the reversible epimerization of D-fructose into D-psicose, D-psicose being the carbon-3 (C3) epimer of D-fructose.

The C. cellulolyticum Dpe is a 33.034 kDa protein of 293 amino acids.

This enzyme is highly specific for D-psicose, but it is also able to catalyse with very low activity the epimerization of D-tagatose [1].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 714
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 D-Psicose 3-epimerase (Dpe) from Clostridium cellulolyticum
-<!-- Add more about the biology of this part here
+This part is an ''E. coli'' codon optimized version of the D-Psicose 3-epimerase (Dpe) from ''Clostridium cellulolyticum'' str. ATCC 35319
+(Uniprot B8I944, [[Part:BBa_K2448021|BBa_K2448021]]).equipped with a strong RBS ([[Part:BBa_K2448019|BBa_K2448019]]) and placed under the control of the pTacI promoter ([[Part:BBa_K864400|BBa_K864400]]).
 ===Usage and Biology===
+D-Psicose 3-epimerase (EC: 5.1.3.30) catalyses the reversible epimerization of D-fructose into D-psicose, D-psicose being the carbon-3 (C3) epimer of D-fructose.
+[[File: T--Evry_Paris-Saclay--EC 5.1.3.30.png|300px]]
+The ''C. cellulolyticum'' Dpe is a 33.034 kDa protein of 293 amino acids.
+This enzyme is highly specific for D-psicose, but it is also able to catalyse with very low activity the epimerization of D-tagatose [1].
 <!-- -->