Difference between revisions of "Part:BBa K1875003:Experience"

(Characterization by iGEM Kyoto 2017)
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==Characterization by ''' [http://2017.igem.org/Team:Kyoto iGEM Kyoto 2017] ''' ==
 
==Characterization by ''' [http://2017.igem.org/Team:Kyoto iGEM Kyoto 2017] ''' ==
We cultured <i>B. xylophilus</i> with <i>S. cerevisiae</i> expressing EGFP of this part from the TDH3 promoter (BBa_K530008). After several days we collected the nematodes and observed them with a fluorescence microscope, and we were able to confirm nematodes emitting green fluorescence throughout the intestinal tract.  
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We cultured <i>B. xylophilus</i> with <i>S. cerevisiae</i> expressing EGFP of this part from the TDH3 promoter (''' [https://parts.igem.org/Part:BBa_K530008  BBa_K530008] ''' ). After several days we collected the nematodes and observed them with a fluorescence microscope (fig 1 ), and we were able to confirm nematodes emitting green fluorescence throughout the intestinal tract.  
 
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<html>
<!-- 腸管がEGFPでぱんぱんのセンチュウの画像-->
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<figure>
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<img src="https://static.igem.org/mediawiki/parts/8/82/B._xEGFP.jpg" alt="<i>B. xylophilus</i> filled with EGFP in its intestine" style="float:center;width:350px;height:250px;">
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        </figure>
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Figure1: <i>B. xylophilus</i> full of EGFP in its esophagus.
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</html>
  
 
===User Reviews===
 
===User Reviews===

Revision as of 11:06, 1 November 2017


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1875003

Characterization by [http://2017.igem.org/Team:Kyoto iGEM Kyoto 2017]

We cultured B. xylophilus with S. cerevisiae expressing EGFP of this part from the TDH3 promoter ( BBa_K530008 ). After several days we collected the nematodes and observed them with a fluorescence microscope (fig 1 ), and we were able to confirm nematodes emitting green fluorescence throughout the intestinal tract.

<i>B. xylophilus</i> filled with EGFP in its intestine
Figure1: B. xylophilus full of EGFP in its esophagus.

User Reviews

UNIQ14202a64e4668774-partinfo-00000001-QINU UNIQ14202a64e4668774-partinfo-00000002-QINU