Difference between revisions of "Part:BBa K2483000"

Revision as of 10:53, 1 November 2017

IAA producing enzymes fused to RNA-binding proteins under control of Pveg2

This part is an upgrade to the part BBa_K515100 (https://parts.igem.org/Part:BBa_K515100) of iGEM Imperial College 2012 producing Indoleacetic acid with the enzymes IaaM (Indoleacetic Acid tryptophan monooxygenase) and IaaH (Indoleacetamide Hydrolase). Here we just fused the IAA enzymes to the RNA-binding proteins (RBPs) MS2 and PP7, respectively.

We used this part to produce Indoleacetic Acid (IAA) in E.coli and increase the efficiency of the reaction by putting them in close proximity to each other (metabolic channelling). This was done with a dCas9 (https://parts.igem.org/Part:BBa_K2483002) scaffold where the RNA-binding proteins bind to aptamer sequences added to the 3'-end of a sgRNA and the sgRNA binds to target cassettes where several of the sgRNA recognition sequences are located behind each other (BBa_K2483005 https://parts.igem.org/Part:BBa_K2483005 and BBa_K2483006 https://parts.igem.org/Part:BBa_K2483006").

For the complete part with sgRNA, dCas9 and the IAA-enzymes, see https://parts.igem.org/Part:BBa_K2483004.

Sequence and Features