Difference between revisions of "Part:BBa K2317001"
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<h2><partinfo>BBa_K2317001 short</partinfo></h2> | <h2><partinfo>BBa_K2317001 short</partinfo></h2> | ||
| − | CbeA-CbtA is one of the Escherichia coli TA systems, and the Toxin, CbtA, has been reported to inhibit the polymerization of bacterial cytoskeletal proteins, MreB and FtsZ.(Fugure 1) | + | <p>CbeA-CbtA is one of the Escherichia coli TA systems, and the Toxin, CbtA, has been reported to inhibit the polymerization of bacterial cytoskeletal proteins, MreB and FtsZ.(Fugure 1)</p> |
[[File:T--Jilin_China--design001.png|550px|thumb|center|Figure 1. The mechanism of CbeA and CbtA(type IV TA system). ]] | [[File:T--Jilin_China--design001.png|550px|thumb|center|Figure 1. The mechanism of CbeA and CbtA(type IV TA system). ]] | ||
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| + | <h1>'''Characterization:'''</h1> | ||
| + | <p>The population and growth condition can be reflected by Abs600, so through the curve of Abs600-time, the effect of CbeA and CbtA towards bacteria can be displayed. | ||
| + | Abs600 values were measured from 0 to 14 hours in two groups and the toxication curves were drawn as shown in Figure 2. The growth rate of vector group bacteria after adding L-Arabinose sped up for a short time and then slowed down. At last, the trend was consistent with that without L-Arabinose. However, TA group, with the addition of L-Arabinose, showed the same OD and Abs600 values from the 4th hour for a while. After that, Abs600 values displayed a little increase. | ||
| + | </p> | ||
| + | [[File:"T-Jilin_China--fg2.png|700px|thumb|center|Figure 2. (A) Different concentrations of L-Arabinose were added into vector group and the growth curve was drawn to made sure the effect of L-Arabinose towards bacteria. According to the results, the addition of L-Arabinose would first slightly enhance and then suppress the growth. However, this effect would disappear after a while. (B) Different concentrations of L-Arabinose were added into TA group and the growth curve was drawn to made sure the effect of CbtA(induced by L-Arabinose) towards bacteria. According to the results, the addition of LArabinose the growth would be stagnated at first and then recover. Fig.]] | ||
| + | Besides, after culturing for 14 hrs, difference in turbidities of bacteria in different groups was visible Figure 3. | ||
| + | [[File:T-Jilin_China--fg3.png|700px|thumb|center|Figure 3. (A) and (B) The two figures were taken to compare the turbidities of TA group after 14 hours od culturing, and the difference could be told by eyes. ]] | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 10:46, 1 November 2017
CbtA
CbeA-CbtA is one of the Escherichia coli TA systems, and the Toxin, CbtA, has been reported to inhibit the polymerization of bacterial cytoskeletal proteins, MreB and FtsZ.(Fugure 1)
Characterization:
The population and growth condition can be reflected by Abs600, so through the curve of Abs600-time, the effect of CbeA and CbtA towards bacteria can be displayed. Abs600 values were measured from 0 to 14 hours in two groups and the toxication curves were drawn as shown in Figure 2. The growth rate of vector group bacteria after adding L-Arabinose sped up for a short time and then slowed down. At last, the trend was consistent with that without L-Arabinose. However, TA group, with the addition of L-Arabinose, showed the same OD and Abs600 values from the 4th hour for a while. After that, Abs600 values displayed a little increase.
Besides, after culturing for 14 hrs, difference in turbidities of bacteria in different groups was visible Figure 3.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]