Difference between revisions of "Part:BBa K530008:Experience"

Revision as of 10:44, 1 November 2017

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Applications of BBa_K530008

Team Kyoto 2017

S. cerevisiae expressing GFP from this TDH3 promoter was cultured and observed with a fluorescence microscope. The strong green fluorescence was observed. (fig 1 )

   </figure>

Figure1: Observation of S. cerevisiae expressing EGFP by this TDH3 promoter with a fluorescence microscope.

B. xylophilus was cultured using this green fluorescent yeast as a bait. After several days, confocal observation confirmed that the intestine of B. xylophilus was filled with EGFP. (fig 2 )

Figure2 : B. xylophilus full of EGFP in its esophagus.

This means that using the TDH3 promoter (BBa_K530008) makes it possible to express a very large amount of EGFP in S. cerevisiae, and that its expression level is sufficient to trace the intestinal tract of nematodes feeding on yeast. The above results indicate that BBa_K530008 and BBa_K1875003 work effectively as a new usage that has not been attempted so far, greatly extending the function of each part.

User Reviews

UNIQ128c0bdd7576a68b-partinfo-00000000-QINU UNIQ128c0bdd7576a68b-partinfo-00000001-QINU

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 <i>S. cerevisiae</i> expressing GFP from this TDH3 promoter was cultured and observed with a fluorescence microscope. The strong green fluorescence was observed. (fig 1 )
-<img src="https://static.igem.org/mediawiki/2017/4/48/GFP_green.jpg">
+<figure>
+	<img src="https://static.igem.org/mediawiki/2017/4/48/GFP_green.jpg" 	alt="Yeast expressing EGFP" 		 style="float:center;width:350px;height:205.5px;">
+    </figure>
 Figure1: Observation of <i>S. cerevisiae</i> expressing EGFP by this TDH3 promoter with a fluorescence microscope.