Difference between revisions of "Part:BBa K2448050:Design"

 
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===Design Notes===
 
===Design Notes===
 
pPsiTac1 is a synthetic promoter derived from pTacI ([[Part:BBa_K864400|BBa_K864400]]) from which we removed the LacO sequence recognized by LacI and replaced it by the consensus binding sequence of a transcription factor, PsiR ([[Part:BBa_K2448006|BBa_K2448006]]). A second consensus binding sequence of PsiR was added before the -35 box.
 
pPsiTac1 is a synthetic promoter derived from pTacI ([[Part:BBa_K864400|BBa_K864400]]) from which we removed the LacO sequence recognized by LacI and replaced it by the consensus binding sequence of a transcription factor, PsiR ([[Part:BBa_K2448006|BBa_K2448006]]). A second consensus binding sequence of PsiR was added before the -35 box.
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===Source===
 
===Source===

Revision as of 10:37, 1 November 2017


pPsiTac2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

pPsiTac1 is a synthetic promoter derived from pTacI (BBa_K864400) from which we removed the LacO sequence recognized by LacI and replaced it by the consensus binding sequence of a transcription factor, PsiR (BBa_K2448006). A second consensus binding sequence of PsiR was added before the -35 box.

Source

DNA synthesis

References