Difference between revisions of "Part:BBa K530008:Experience"
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<i>S. cerevisiae</i> expressing GFP from this TDH 3 promoter was cultured and observed with a fluorescence microscope. The strong green fluorescence was observed. (fig 1 ) | <i>S. cerevisiae</i> expressing GFP from this TDH 3 promoter was cultured and observed with a fluorescence microscope. The strong green fluorescence was observed. (fig 1 ) | ||
| − | + | [[File:GFP yeast.jpeg|500px|thumb|center| '''Figure1: Observation of <i>S. cerevisiae</i> expressing EGFP by this TDH3 promoter with a fluorescence microscope. ''']] | |
| − | Figure1 : Observation of <i>S. cerevisiae</i> expressing EGFP by this TDH3 promoter with a fluorescence microscope. | + | |
<i>B. xylophilus</i> was cultured using this green fluorescent yeast as a bait. After several days, confocal observation confirmed that the intestine of <i>B. xylophilus</i> was filled with EGFP (photo or link). (fig 2 ) | <i>B. xylophilus</i> was cultured using this green fluorescent yeast as a bait. After several days, confocal observation confirmed that the intestine of <i>B. xylophilus</i> was filled with EGFP (photo or link). (fig 2 ) | ||
| − | + | [[File:アフリカツメガエル結果injection.png|500px|thumb|center| '''Figure2 : <i>B. xylophilus</i> full of EGFP in its esophagus. | |
| − | Figure2 : <i>B. xylophilus</i> full of EGFP in its esophagus. | + | |
| + | ''']] | ||
This means that using the TDH3 promoter (BBa_K530008) makes it possible to express a very large amount of EGFP in <i>S. cerevisiae</i>, and that its expression level is sufficient to trace the intestinal tract of nematodes feeding on yeast. The above results indicate that ''' [https://parts.igem.org/Part:BBa_K530008 BBa_K530008] ''' and ''' [https://parts.igem.org/Part:BBa_K1875003 BBa_K1875003] ''' work effectively as a new usage that has not been attempted so far, greatly extending the function of each part. | This means that using the TDH3 promoter (BBa_K530008) makes it possible to express a very large amount of EGFP in <i>S. cerevisiae</i>, and that its expression level is sufficient to trace the intestinal tract of nematodes feeding on yeast. The above results indicate that ''' [https://parts.igem.org/Part:BBa_K530008 BBa_K530008] ''' and ''' [https://parts.igem.org/Part:BBa_K1875003 BBa_K1875003] ''' work effectively as a new usage that has not been attempted so far, greatly extending the function of each part. | ||
Revision as of 10:19, 1 November 2017
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Applications of BBa_K530008
Team Kyoto 2017
S. cerevisiae expressing GFP from this TDH 3 promoter was cultured and observed with a fluorescence microscope. The strong green fluorescence was observed. (fig 1 )
B. xylophilus was cultured using this green fluorescent yeast as a bait. After several days, confocal observation confirmed that the intestine of B. xylophilus was filled with EGFP (photo or link). (fig 2 )
This means that using the TDH3 promoter (BBa_K530008) makes it possible to express a very large amount of EGFP in S. cerevisiae, and that its expression level is sufficient to trace the intestinal tract of nematodes feeding on yeast. The above results indicate that BBa_K530008 and BBa_K1875003 work effectively as a new usage that has not been attempted so far, greatly extending the function of each part.
User Reviews
UNIQaa58433f55287418-partinfo-00000000-QINU UNIQaa58433f55287418-partinfo-00000001-QINU