Difference between revisions of "Part:BBa K2194002"
Catdunaway (Talk | contribs) |
Catdunaway (Talk | contribs) |
||
Line 3: | Line 3: | ||
<partinfo>BBa_K2194002 short</partinfo> | <partinfo>BBa_K2194002 short</partinfo> | ||
− | The | + | The <i>Pgntk</i> promoter is a native <i>Escherichia coli</i> negative feedback membrane stress promoter. BioBrick BBa_K2194002 contains the 200 bp upstream of the <i>gntK</i> gene in <i>Escherichia coli</i>, which has been previously experimentally shown to incorporate the full <i>Pgntk</i> promoter region. [1] |
Revision as of 09:59, 1 November 2017
PgntK Negative Feedback Membrane Stress Promoter
The Pgntk promoter is a native Escherichia coli negative feedback membrane stress promoter. BioBrick BBa_K2194002 contains the 200 bp upstream of the gntK gene in Escherichia coli, which has been previously experimentally shown to incorporate the full Pgntk promoter region. [1]
Biology
[1] Boyarskiy, Sergey et al. “Transcriptional Feedback Regulation of Efflux Protein Expression for Increased Tolerance to and Production of N-Butanol.” Metabolic Engineering 33 (2016): 130–137. Web. 28 Oct. 2017.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 14
Illegal BsaI.rc site found at 216