Difference between revisions of "Part:BBa K2294007"

Revision as of 09:55, 1 November 2017

Synthtic minimal Galactose induced promoter + Kozak sequence

A short synthtetic promoter which can be induced by the addition of Galactose. This promoter contains already a Kozak sequence for the expression of proteins. The promoter is only 160 bp long.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Usage and Biology

This promoter is build up fully modular. The promoter starts with an GAL4 binding site derived from GAL1. After this trnaskription factor binding site a neutral spacer is placed to avoid steric hindrances between GAL4 and the TATA-box-binding- proteins of the preinitiation complex.

Promoter characterization

The BOKU-Vienna Team 2017 submitted and characterized this part by measuring the GFP expression using flow cytometry. For detais go to the Experience page.

References

Redden, H., & Alper, H. S. (2015). The development and characterization of synthetic minimal yeast promoters. Nature Communications, 6, 7810. https://doi.org/10.1038/ncomms8810

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 ===Usage and Biology===
-This promoter is build up fully modular. The promoter starts with an GAL4 binding site derived from <i>GAL1</i>. After this trnaskription factor binding site a neutral spacer is placed to avoid steric hindrances between GAL4 and the TATA-box-binding- proteins of the preinitiation complex. The core element following the TATA-box contains all sequence components necessary for transcription initiation.
+This promoter is build up fully modular. The promoter starts with an GAL4 binding site derived from <i>GAL1</i>. After this trnaskription factor binding site a neutral spacer is placed to avoid steric hindrances between GAL4 and the TATA-box-binding- proteins of the preinitiation complex.
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