Difference between revisions of "Part:BBa I732103"
Line 3: Line 3:
 
[[Image:USTC_1l1m_bio_r_500.jpg|thumb|150px|'''Figure 1''' Solution structure of a dimer of Lac repressor DNA-binding domain complexed to its natural operator O1 ([http://www.rcsb.org/pdb/explore.do?structureId=1L1M From RCSB])]]
 
[[Image:USTC_1l1m_bio_r_500.jpg|thumb|150px|'''Figure 1''' Solution structure of a dimer of Lac repressor DNA-binding domain complexed to its natural operator O1 ([http://www.rcsb.org/pdb/explore.do?structureId=1L1M From RCSB])]]
  
−
The sequence produce LaLa repressor protein which is based on natural lacR with only several point mutations. Standard BioBrick Prefix and Suffix are added to both ends of this part for standard assembly.
+
[[Image:USTC_MullerRepression.png|thumb|center|300px|'''Figure I''' Repression Metrix(RM):The repression matrix reveals the binding affinity of different repressor candidates with various specific promoters by different colors. The deeper the red will be, the higher the repression will appear. While the lighter the blue is the weaker the repression will show. This Matrix are not directly from literatures but from the data by previous research work.]]
  
  
−
===Usage and Biology===
+
The sequence produce LRLb repressor protein which is based on natural lacR with only several point mutations. Standard BioBrick Prefix and Suffix are added to both ends of this part for standard assembly.
  
−
LaLa could strongly inhibit its corresponding operator which is based on natural lac operator with only several point mutations while it has also weak inhibition to natural lac operator.
+
 
 +
===Usage and Biology===
 +
Refer to the Repression Matrix on the above, we picked up 4 well-performing repressors as candidates to do our study; they are LRLa, LRLb, LRLc, LRLd.  
  
 
(''Reference'':
 
(''Reference'':
Line 14: Line 16:
 
2.Lehming, N.; Sartorius, J.; Oehler, S.; von Wilcken-Bergmann, B. & Müller-Hill, B. Recognition helices of lac and lambda repressor are oriented in opposite directions and recognize similar DNA sequences. PNAS, 1988, 85, 7947-7951
 
2.Lehming, N.; Sartorius, J.; Oehler, S.; von Wilcken-Bergmann, B. & Müller-Hill, B. Recognition helices of lac and lambda repressor are oriented in opposite directions and recognize similar DNA sequences. PNAS, 1988, 85, 7947-7951
 
3. Sartorius, J.; Lehming, N.; Kisters, B.; von Wilcken-Bergmann, B. & Müller-Hill, B. (1989), 'lac repressor mutants with double or triple exchanges in the recognition helix bind specifically to lac operator variants with multiple exchanges.', EMBO J 8(4), 1265--1270.)
 
3. Sartorius, J.; Lehming, N.; Kisters, B.; von Wilcken-Bergmann, B. & Müller-Hill, B. (1989), 'lac repressor mutants with double or triple exchanges in the recognition helix bind specifically to lac operator variants with multiple exchanges.', EMBO J 8(4), 1265--1270.)
−
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 07:00, 26 October 2007

LacI

Figure 1 Solution structure of a dimer of Lac repressor DNA-binding domain complexed to its natural operator O1 ([http://www.rcsb.org/pdb/explore.do?structureId=1L1M From RCSB])
Figure I Repression Metrix(RM):The repression matrix reveals the binding affinity of different repressor candidates with various specific promoters by different colors. The deeper the red will be, the higher the repression will appear. While the lighter the blue is the weaker the repression will show. This Matrix are not directly from literatures but from the data by previous research work.


The sequence produce LRLb repressor protein which is based on natural lacR with only several point mutations. Standard BioBrick Prefix and Suffix are added to both ends of this part for standard assembly.


Usage and Biology

Refer to the Repression Matrix on the above, we picked up 4 well-performing repressors as candidates to do our study; they are LRLa, LRLb, LRLc, LRLd.

(Reference: 1.Lewis, M. (2005), 'The lac repressor.', C R Biol 328 (6), 521--548. 2.Lehming, N.; Sartorius, J.; Oehler, S.; von Wilcken-Bergmann, B. & Müller-Hill, B. Recognition helices of lac and lambda repressor are oriented in opposite directions and recognize similar DNA sequences. PNAS, 1988, 85, 7947-7951 3. Sartorius, J.; Lehming, N.; Kisters, B.; von Wilcken-Bergmann, B. & Müller-Hill, B. (1989), 'lac repressor mutants with double or triple exchanges in the recognition helix bind specifically to lac operator variants with multiple exchanges.', EMBO J 8(4), 1265--1270.)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]