Difference between revisions of "Part:BBa K2457001"
Giomaklouf (Talk | contribs) |
|||
| Line 10: | Line 10: | ||
===Design=== | ===Design=== | ||
| + | [[File:BBa K2457001 circuit.png]] | ||
| + | |||
| + | Figure 1: BBa_K2457001 circuit. | ||
===Characterization=== | ===Characterization=== | ||
| Line 15: | Line 18: | ||
[[File:BBa K2457001 electropherogram.png]] | [[File:BBa K2457001 electropherogram.png]] | ||
| − | Figure | + | Figure 2:Sequencing electropherogram from BBa_K2457001. |
[[File:BBa K2457001 aligment.png]] | [[File:BBa K2457001 aligment.png]] | ||
| − | Figure | + | Figure 3:Alignment of the designed sequence and our final construction from BBa_K2457001. |
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 09:33, 1 November 2017
Standardized Cas9 coding sequence + B0015 terminator
This brick was engineered to be a building block of the standard BioBrick vector for one-step bacterial genome engineering: the ([http://2017.igem.org/Team:Amazonas_Brazil pCRISPeasy]). BBa_K2457001 is composed of the Cas9 coding sequence from Streptococcus pyogenes + BBa_B0015 double terminator. It was rationally designed to be interchangeable AND standardized, providing a BioBrick to attend multiple SynBio purposes. For that matter, the RBS and promoter are easily switchable through 3A Assembly. It’s also standard restriction enzymes safe for all RFCs assemblies.
[[File:]]
Usage and Biology
Design
Figure 1: BBa_K2457001 circuit.
Characterization
Figure 2:Sequencing electropherogram from BBa_K2457001.
Figure 3:Alignment of the designed sequence and our final construction from BBa_K2457001.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]