Difference between revisions of "Part:BBa K2365052"
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<partinfo>BBa_K2365052 short</partinfo>
 
<partinfo>BBa_K2365052 short</partinfo>
  
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Between the TPI1 promoter and CYC1 terminator,having the restriction enzyme cutting site.And you can insert the gene if you want.And it is inserted into the expression vector pRS423 and inserted into GFP (S65T), then transformed into Saccharomyces cerevisiae SEY6210 to test the fluorescence intensity of 440-550nm
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Between the TPI1 promoter and CYC1 terminator,having the restriction enzyme cutting site.And you can insert the gene if you want.
  
 
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<partinfo>BBa_K2365052 parameters</partinfo>
 
<partinfo>BBa_K2365052 parameters</partinfo>
 
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We used reserved restriction enzyme cutting site to inserted GPF (S6GT) between promoter and termiator as the index of promoter test. After 30 hours incubation, we measured the fluorescence intensity of the transformed yeast at 440 nm to 530 nm
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[[File: U-disk test.jpg|500px|center]]
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[[File:酵母荧光.jpg|700px|center]]

Revision as of 09:17, 1 November 2017


TPI1 promotor-CYC1 terminator

Between the TPI1 promoter and CYC1 terminator,having the restriction enzyme cutting site.And you can insert the gene if you want.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 246
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 318


We used reserved restriction enzyme cutting site to inserted GPF (S6GT) between promoter and termiator as the index of promoter test. After 30 hours incubation, we measured the fluorescence intensity of the transformed yeast at 440 nm to 530 nm

U-disk test.jpg
酵母荧光.jpg