Difference between revisions of "Part:BBa I732100"
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The lac repressor is a DNA-binding protein which inhibits the expression of genes coding for proteins involved in the metabolism of lactose in bacteria. There are three distinct regions in the protein. The headpiece, a fragment contains approximately 51 amino acids from its N-terminal together with a helix-turn-helix motif, is the only region that serves to bind DNA. Figure I shows the NMR structure of the complex with two headpieces of the lac repressor and its nature operator. | The lac repressor is a DNA-binding protein which inhibits the expression of genes coding for proteins involved in the metabolism of lactose in bacteria. There are three distinct regions in the protein. The headpiece, a fragment contains approximately 51 amino acids from its N-terminal together with a helix-turn-helix motif, is the only region that serves to bind DNA. Figure I shows the NMR structure of the complex with two headpieces of the lac repressor and its nature operator. | ||
| − | (''Reference'':Lewis, M. (2005), 'The lac repressor.', C R Biol 328 (6), 521--548.) | + | (''Reference'': |
| + | 1.Lewis, M. (2005), 'The lac repressor.', C R Biol 328 (6), 521--548. | ||
| + | 2.Lehming, N.; Sartorius, J.; Oehler, S.; von Wilcken-Bergmann, B. & Müller-Hill, B. Recognition helices of lac and lambda repressor are oriented in opposite directions and recognize similar DNA sequences. PNAS, 1988, 85, 7947-7951 | ||
| + | 3. Sartorius, J.; Lehming, N.; Kisters, B.; von Wilcken-Bergmann, B. & Müller-Hill, B. (1989), 'lac repressor mutants with double or triple exchanges in the recognition helix bind specifically to lac operator variants with multiple exchanges.', EMBO J 8(4), 1265--1270. ) | ||
Latest revision as of 06:29, 26 October 2007
LacI
The sequence produce LacI repressor protein.Standard BioBrick Prefix and Suffix are added to both ends of this part for standard assembly.
Usage and Biology
The lac repressor is a DNA-binding protein which inhibits the expression of genes coding for proteins involved in the metabolism of lactose in bacteria. There are three distinct regions in the protein. The headpiece, a fragment contains approximately 51 amino acids from its N-terminal together with a helix-turn-helix motif, is the only region that serves to bind DNA. Figure I shows the NMR structure of the complex with two headpieces of the lac repressor and its nature operator.
(Reference: 1.Lewis, M. (2005), 'The lac repressor.', C R Biol 328 (6), 521--548. 2.Lehming, N.; Sartorius, J.; Oehler, S.; von Wilcken-Bergmann, B. & Müller-Hill, B. Recognition helices of lac and lambda repressor are oriented in opposite directions and recognize similar DNA sequences. PNAS, 1988, 85, 7947-7951 3. Sartorius, J.; Lehming, N.; Kisters, B.; von Wilcken-Bergmann, B. & Müller-Hill, B. (1989), 'lac repressor mutants with double or triple exchanges in the recognition helix bind specifically to lac operator variants with multiple exchanges.', EMBO J 8(4), 1265--1270. )
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]