Difference between revisions of "Part:BBa K2365039"
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We construct the device：ADH1 promoter+GFP+CYC1 terminator.ADH1 promoter is a truncated promoter, GFP is a mutant. These is a invariable sequence upstream of GFP coding sequence. | We construct the device：ADH1 promoter+GFP+CYC1 terminator.ADH1 promoter is a truncated promoter, GFP is a mutant. These is a invariable sequence upstream of GFP coding sequence. | ||
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The promoter of yeast alcohol dehydrogenase (ADH1) is widely used for the expression of heterologous genes in Saccharomyces cerevisiae. We tested the expression of GFP proteins in five transformants containing the ADH1 promoter. Our data shows that there are some differences in the fluorescence intensity of different yeast transformants with ADH1 promoter. The variation trends of promoter strengths from early-log to stationary phase varied in different transformants. Individual transformants colonies have different expression efficiency. We will test this promoter for more than three times. | The promoter of yeast alcohol dehydrogenase (ADH1) is widely used for the expression of heterologous genes in Saccharomyces cerevisiae. We tested the expression of GFP proteins in five transformants containing the ADH1 promoter. Our data shows that there are some differences in the fluorescence intensity of different yeast transformants with ADH1 promoter. The variation trends of promoter strengths from early-log to stationary phase varied in different transformants. Individual transformants colonies have different expression efficiency. We will test this promoter for more than three times. | ||
Revision as of 08:40, 1 November 2017
ADH1 promoter
AHD1 Yeast promoter Constitutive expression: Alcohol dehydrogenase I promoter, full length version is very strong which can promote high expression. Truncated promoters are constitutive and have low expression. Widely exist in human and animal liver, plant and microbial cells as a key enzyme in short-chain alcohol metabolism. Glucose can promote the presence of expression, in many physiological processes play an important role.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Improved design:
We construct the device：ADH1 promoter+GFP+CYC1 terminator.ADH1 promoter is a truncated promoter, GFP is a mutant. These is a invariable sequence upstream of GFP coding sequence.
The promoter of yeast alcohol dehydrogenase (ADH1) is widely used for the expression of heterologous genes in Saccharomyces cerevisiae. We tested the expression of GFP proteins in five transformants containing the ADH1 promoter. Our data shows that there are some differences in the fluorescence intensity of different yeast transformants with ADH1 promoter. The variation trends of promoter strengths from early-log to stationary phase varied in different transformants. Individual transformants colonies have different expression efficiency. We will test this promoter for more than three times.