Difference between revisions of "Part:BBa K2467002:Design"

 
 
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
native wildtype promoter sequence of the Escherichia coli proU operon
+
consensus promoter sequence of the Escherichia coli proU operon
  
 
===References===
 
===References===
 +
 +
Lucht JM, Bremer E. 1990. Characterization of Mutations Affecting the Osmoregulated proU Promoter of Escherichia coli and Identification of 5' Sequences Required for High-Level Expression. J Bactereol. 173:801-809.

Latest revision as of 07:52, 1 November 2017


Consensus proU-B0034-mRFP-B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 833
    Illegal AgeI site found at 945
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sensor will induce expression of RFP when osmolarity is high. The sensor can be utilized to measure salt concentrations to determine if the SRB can survive.


Source

consensus promoter sequence of the Escherichia coli proU operon

References

Lucht JM, Bremer E. 1990. Characterization of Mutations Affecting the Osmoregulated proU Promoter of Escherichia coli and Identification of 5' Sequences Required for High-Level Expression. J Bactereol. 173:801-809.