Difference between revisions of "Part:BBa K2467002"

Latest revision as of 07:51, 1 November 2017

Consensus proU-B0034-mRFP-B0015

This sensor employs a consensus promoter that was designed to incorporate several mutations to increase expression in response to increases in osmolarity. The sensor will induce expression of RFP when osmolarity is high. The sensor can be utilized to measure salt concentrations to determine if the SRB can survive. The promoter can then be used to produce the dispersin enzyme to degrade the biofilm where the SRB accumulate and corrode the pipes.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 833
Illegal AgeI site found at 945
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2467002 short</partinfo>
-First the promoter is tested by making a sensor with a fluorescent protein reporter. The sensor will induce expression of RFP when osmolarity is high. The sensor can be utilized to measure salt concentrations to determine if  the SRB can survive.  The promoter can then be used to produce the dispersin enzyme to degrade the biofilm where the SRB accumulate and corrode the pipes.
+This sensor employs a consensus promoter that was designed to incorporate several mutations to increase expression in response to increases in osmolarity. The sensor will induce expression of RFP when osmolarity is high. The sensor can be utilized to measure salt concentrations to determine if  the SRB can survive.  The promoter can then be used to produce the dispersin enzyme to degrade the biofilm where the SRB accumulate and corrode the pipes.
 <!-- Add more about the biology of this part here