Difference between revisions of "Part:BBa K2384006"
Line 7: Line 7:
  
  
−
<!-- Add more about the biology of this part here
+
 
 +
<b>Optimization Report</b>
 +
 
 +
1.Codon Used Adjustment
 +
The best value is 1 for sequence optimization.
 +
 
 +
 
 +
<table><tr>
 +
<th>[[Image:T--FAFU-CHINA--cspc1.png|thumb|400px|'''Before Codon Adjustment''']]</th>
 +
</tr></table>
 +
 
 +
<table><tr>
 +
<th>[[Image:T--FAFU-CHINA--cspc2.png|thumb|400px|'''After Codon Adjustment''']]</th>
 +
</tr></table>
 +
 
 +
 
 +
2.Codon Used Distribution
 +
Show the relative codon used distribution
 +
 
 +
<table><tr>
 +
<th>[[Image:T--FAFU-CHINA--cspc3.png|thumb|400px|'''Before Optimization''']]</th>
 +
</tr></table>
 +
 
 +
<table><tr>
 +
<th>[[Image:T--FAFU-CHINA--cspc4.png|thumb|400px|'''After Optimization''']]</th>
 +
</tr></table>
 +
 
 +
3.GC Content:
 +
The comparison of GC content between original sequence and optimized sequence
 +
 
 +
<table><tr>
 +
<th>[[Image:T--FAFU-CHINA--cspc5.png|thumb|400px|'''Before Optimization''']]</th>
 +
</tr></table>
 +
 
 +
<table><tr>
 +
<th>[[Image:T--FAFU-CHINA--cspc6.png|thumb|400px|'''After Optimization''']]</th>
 +
</tr></table>
 +
 
 +
4.Restriction Enzyme
 +
 
 +
<table><tr>
 +
<th>[[Image:T--FAFU-CHINA--cspc7-1.png|thumb|400px|'''After Optimization''']]</th>
 +
</tr></table>
 +
 
 +
 
 
===Usage and Biology===
 
===Usage and Biology===
  
−
<!-- -->
+
 
 +
<table><tr>
 +
<th>[[Image:T--FAFU-CHINA--cspc8.png|thumb|400px|'''Freeze/thaw cycle survival rate of Bacillus megaterium containing an empty vector (red line), a vector carrying CspC gene (blue line).''']]</th>
 +
</tr></table>
 +
 
 
<span class='h3bb'>Sequence and Features
 
<span class='h3bb'>Sequence and Features
 
<partinfo>BBa_K2384006 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2384006 SequenceAndFeatures</partinfo>

Revision as of 07:23, 1 November 2017


Pveg+RBS+CspC+6*HIS+T1 terminator+T7 terminator


This is an improve part.This gene is a cold resistant gene from the Arctic cryophile Polaribacter irgensii.It can largely increase the cold resistance of bacterias.CDS sequence optimization in (BBa_K538004) is better suited for expression in Bacillus megaterium and we add a 6*His tag at the C end.After that, the promoter (BBa_K143012) and RBS (BBa_K090505) were combined.


Optimization Report

1.Codon Used Adjustment The best value is 1 for sequence optimization.


Before Codon Adjustment
After Codon Adjustment


2.Codon Used Distribution Show the relative codon used distribution

Before Optimization
After Optimization

3.GC Content: The comparison of GC content between original sequence and optimized sequence

Before Optimization
After Optimization

4.Restriction Enzyme

After Optimization


Usage and Biology

Freeze/thaw cycle survival rate of Bacillus megaterium containing an empty vector (red line), a vector carrying CspC gene (blue line).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]