Difference between revisions of "Part:BBa K2457001"

Revision as of 04:37, 1 November 2017

Standardized Cas9 coding sequence + B0015 terminator

This brick was engineered to be a building block of the standard BioBrick vector for one-step bacterial genome engineering: the ([http://2017.igem.org/Team:Amazonas_Brazil pCRISPeasy]). BBa_K2457001 is composed of the Cas9 coding sequence from Streptococcus pyogenes + BBa_B0015 double terminator. It was rationally designed to be interchangeable AND standardized, providing a BioBrick to attend multiple SynBio purposes. For that matter, the RBS and promoter are easily switchable through 3A Assembly. It’s also standard restriction enzymes safe for all RFCs assemblies.

File:BBa K2457001

Usage and Biology

Design

Characterization

Figure 1:Sequencing electropherogram from BBa_K2457001.

Figure 2:Alignment of the designed sequence and our final construction from BBa_K2457001.

Sequence and Features