Difference between revisions of "Part:BBa K2194001:Design"

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===Design Notes===
 
===Design Notes===
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[update]
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Primers used to PCR amplify <i>sbp</i> from the <i>Escherichia coli</i> MG1655 genome<sup>NOTE</sup>: <p>
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+
5' <b>CGATCGTCTCACTCGA</b>ATGAACAAGTGGGGCGTAG3'</p><p>
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5' <b>GCATCGTCTCACTCTGCCA</b>TCAGCGTTTGCTGATCTG3'</p>
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<br>
  
 +
NOTE: The primer overhangs are <b>bolded</b>.
  
 
===Source===
 
===Source===

Revision as of 02:40, 1 November 2017


Sulfate Binding Protein (sbp) cds


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3
    Illegal BsaI.rc site found at 1006


Design Notes

Primers used to PCR amplify sbp from the Escherichia coli MG1655 genomeNOTE:

5' CGATCGTCTCACTCGAATGAACAAGTGGGGCGTAG3'

5' GCATCGTCTCACTCTGCCATCAGCGTTTGCTGATCTG3'


NOTE: The primer overhangs are bolded.

Source

Escherichia coli genomic sequence

References