Difference between revisions of "Part:BBa K2213012"
Line 23: Line 23:
 
<br>
 
<br>
 
https://static.igem.org/mediawiki/2017/4/4f/LowSMNLKDAPIcells.png<br>
 
https://static.igem.org/mediawiki/2017/4/4f/LowSMNLKDAPIcells.png<br>
 +
<b>Figure 2.</b> Fluorescence microscopy images of promoter-PduD associated mCherry and DAPI stained polyphosphate.<br>
 
<br>
 
<br>
 
As shown, there is a heterogeneous distribution of mCherry signal indicating successful tag localisation and by proxy successful expression of Eut subunits.
 
As shown, there is a heterogeneous distribution of mCherry signal indicating successful tag localisation and by proxy successful expression of Eut subunits.
 
<br>
 
<br>

Revision as of 02:20, 1 November 2017


LacUV5_EutS_Tet_EutMN

/

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1260
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 4125
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3451


Function:
This part was expressed alongside EutLK-Low-PduD-mCherry-PPK (https://parts.igem.org/Part:BBa_K2213013) to form EutSMNLK+Low_PduD+PPK which was characterized as follows.

A 24 hour induction was DAPI stained to determine microcompartment formation and tag localisation.

LowSMNLKDAPIcells.png
Figure 2. Fluorescence microscopy images of promoter-PduD associated mCherry and DAPI stained polyphosphate.

As shown, there is a heterogeneous distribution of mCherry signal indicating successful tag localisation and by proxy successful expression of Eut subunits.