Difference between revisions of "Part:BBa K2440032"
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MiR-489 was firstly found in the adult fish’s brain and eye.<sup>1</sup> | MiR-489 was firstly found in the adult fish’s brain and eye.<sup>1</sup> | ||
| − | Compared to the corresponding non-tumor tissues, miR-489 was frequently downregulated in colorectal cancer (CRC). By Kaplan-Meier analysis, lower CRC recurrence free survival years in the group with elevated miR-489 expression than those with lower miR-489 expression. Moreover, miR-489 targets the 3'-UTR of the HDAC7 transcript and downregulates its expression, and HDAC7 expression promoted tumor cell proliferation and invasion. In conclusion, miR-489 suppresses tumor invasion and metastasis in CRC by targeting HDAC7.<sup> | + | Compared to the corresponding non-tumor tissues, miR-489 was frequently downregulated in colorectal cancer (CRC). By Kaplan-Meier analysis, lower CRC recurrence free survival years in the group with elevated miR-489 expression than those with lower miR-489 expression. Moreover, miR-489 targets the 3'-UTR of the HDAC7 transcript and downregulates its expression, and HDAC7 expression promoted tumor cell proliferation and invasion. In conclusion, miR-489 suppresses tumor invasion and metastasis in CRC by targeting HDAC7.<sup>2</sup> |
| − | Moreover, miR-489 can inhibit proliferation, cell cycle progression and induces apoptosis of glioma cells via targeting SPIN1-mediated PI3K/AKT pathway.<sup> | + | Moreover, miR-489 can inhibit proliferation, cell cycle progression and induces apoptosis of glioma cells via targeting SPIN1-mediated PI3K/AKT pathway.<sup>3</sup> |
===Sequence and Features=== | ===Sequence and Features=== | ||
Revision as of 01:23, 1 November 2017
miR-489 target sequence
It is the target sequence of miR-489, a modularized DNA part from a set of chemically synthetic oligo DNA library.
Usage and Biology
MiRNA locker assembled by using this modularized DNA part was able to bind miR-489 in an Ago2 dependent manner, that is, knockdown of miR-489 was achieved by transfecting cells with miRNA locker.
MiR-489 was firstly found in the adult fish’s brain and eye.1
Compared to the corresponding non-tumor tissues, miR-489 was frequently downregulated in colorectal cancer (CRC). By Kaplan-Meier analysis, lower CRC recurrence free survival years in the group with elevated miR-489 expression than those with lower miR-489 expression. Moreover, miR-489 targets the 3'-UTR of the HDAC7 transcript and downregulates its expression, and HDAC7 expression promoted tumor cell proliferation and invasion. In conclusion, miR-489 suppresses tumor invasion and metastasis in CRC by targeting HDAC7.2
Moreover, miR-489 can inhibit proliferation, cell cycle progression and induces apoptosis of glioma cells via targeting SPIN1-mediated PI3K/AKT pathway.3
Sequence and Features
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Experimental Validation
This part is sequenced as correct after construction.