Difference between revisions of "Part:BBa K2440031"

(Usage and Biology)
(Usage and Biology)
Line 9: Line 9:
 
MiRNA locker assembled by using this modularized DNA part was able to bind miR-155 in an Ago2 dependent manner, that is, knockdown of miR-155 was achieved by transfecting cells with miRNA locker.
 
MiRNA locker assembled by using this modularized DNA part was able to bind miR-155 in an Ago2 dependent manner, that is, knockdown of miR-155 was achieved by transfecting cells with miRNA locker.
  
Dre-miR155 was firstly found in zebrafish.1
+
Dre-miR155 was firstly found in zebrafish.<sup>1</sup>
  
MiR-155-5p are highly expressed in the heart and regulate cardiac development. What’s more, miR-155-5p was overexpressed in trisomic hearts2.
+
MiR-155-5p are highly expressed in the heart and regulate cardiac development. What’s more, miR-155-5p was overexpressed in trisomic hearts.<sup>2</sup>
  
MiR-155 downregulation in NK cells of IA patients impaired IFN-γ production by targeting SOCS1, which may contribute to immune dysfunction during CHB infection. Additionally, baseline miR-155 expression could predict the treatment response to telbivudine therapy3.
+
MiR-155 downregulation in NK cells of IA patients impaired IFN-γ production by targeting SOCS1, which may contribute to immune dysfunction during CHB infection. Additionally, baseline miR-155 expression could predict the treatment response to telbivudine therapy.<sup>3</sup>
  
 
===Sequence and Features===
 
===Sequence and Features===

Revision as of 01:19, 1 November 2017


miR-155 target sequence

It is the target sequence of miR-155, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR-155 in an Ago2 dependent manner, that is, knockdown of miR-155 was achieved by transfecting cells with miRNA locker.

Dre-miR155 was firstly found in zebrafish.1

MiR-155-5p are highly expressed in the heart and regulate cardiac development. What’s more, miR-155-5p was overexpressed in trisomic hearts.2

MiR-155 downregulation in NK cells of IA patients impaired IFN-γ production by targeting SOCS1, which may contribute to immune dysfunction during CHB infection. Additionally, baseline miR-155 expression could predict the treatment response to telbivudine therapy.3

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Experimental Validation

This part is sequenced as correct after construction.