Difference between revisions of "Part:BBa K2440030"

Revision as of 01:18, 1 November 2017

miR-152 target sequence

It is the target sequence of miR-152, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR-152 in an Ago2 dependent manner, that is, knockdown of miR-152 was achieved by transfecting cells with miRNA locker.

Dre-miR-152 is a novel miRNA founded in zebrafish.¹

It was found that miR-152 was underexpressed in human osteosarcoma (OS) tissues and cell lines. Decreased miR-152 was inversely correlated with lymph-node metastasis and advanced clinical stage. Overexpression of miR-152 significantly inhibited cell proliferation, colony formation, migration and invasion of OS cells. Bioinformatics analyses showed that miR-152 directly targeted E2F transcription factor 3 (E2F3), as further confirmed by a dual-luciferase reporter assay. Dre-miR-152 may be a therapeutic target for OS.²

Moreover, MiR-152 regulated cell proliferation and apoptosis of glioma mediated by Runx2, while the mechanism of down regulated miR-152 in glioma tissues and cells was its hypermethylation.³

Sequence and Features

Sequence and Features

Experimental Validation

This part is sequenced as correct after construction.

Reference

1.The developmental miRNA profiles of zebrafish as determined by small RNA cloning. Chen PY, Manninga H, Slanchev K, Chien M, Russo JJ, Ju J, Sheridan R, John B, Marks DS, Gaidatzis D, Sander C, Zavolan M, Tuschl T Genes Dev. 19:1288-1293(2005).

2.MicroRNA-152 Suppresses Human Osteosarcoma Cell Proliferation and Invasion by Targeting E2F Transcription Factor 3. Ma C, Han J, Dong D, Wang N.

3.MiR-152 regulated glioma cell proliferation and apoptosis via Runx2 mediated by DNMT1. Zhang P, Sun H, Yang B, Luo W, Liu Z, Wang J, Zuo Y.