Difference between revisions of "Part:BBa K2440030"

(Usage and Biology)
(Usage and Biology)
Line 9: Line 9:
 
MiRNA locker assembled by using this modularized DNA part was able to bind miR-152 in an Ago2 dependent manner, that is, knockdown of miR-152 was achieved by transfecting cells with miRNA locker.
 
MiRNA locker assembled by using this modularized DNA part was able to bind miR-152 in an Ago2 dependent manner, that is, knockdown of miR-152 was achieved by transfecting cells with miRNA locker.
  
Dre-miR-152 is a novel miRNA founded in zebrafish.1
+
Dre-miR-152 is a novel miRNA founded in zebrafish.<sup>1</sup>
  
It was found that miR-152 was underexpressed in human osteosarcoma (OS) tissues and cell lines. Decreased miR-152 was inversely correlated with lymph-node metastasis and advanced clinical stage. Overexpression of miR-152 significantly inhibited cell proliferation, colony formation, migration and invasion of OS cells. Bioinformatics analyses showed that miR-152 directly targeted E2F transcription factor 3 (E2F3), as further confirmed by a dual-luciferase reporter assay. Dre-miR-152 may be a therapeutic target for OS2.
+
It was found that miR-152 was underexpressed in human osteosarcoma (OS) tissues and cell lines. Decreased miR-152 was inversely correlated with lymph-node metastasis and advanced clinical stage. Overexpression of miR-152 significantly inhibited cell proliferation, colony formation, migration and invasion of OS cells. Bioinformatics analyses showed that miR-152 directly targeted E2F transcription factor 3 (E2F3), as further confirmed by a dual-luciferase reporter assay. Dre-miR-152 may be a therapeutic target for OS.<sup>1</sup>
  
Moreover, MiR-152 regulated cell proliferation and apoptosis of glioma mediated by Runx2, while the mechanism of down regulated miR-152 in glioma tissues and cells was its hypermethylation3.
+
Moreover, MiR-152 regulated cell proliferation and apoptosis of glioma mediated by Runx2, while the mechanism of down regulated miR-152 in glioma tissues and cells was its hypermethylation.<sup>1</sup>
  
 
===Sequence and Features===
 
===Sequence and Features===

Revision as of 01:17, 1 November 2017


miR-152 target sequence

It is the target sequence of miR-152, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR-152 in an Ago2 dependent manner, that is, knockdown of miR-152 was achieved by transfecting cells with miRNA locker.

Dre-miR-152 is a novel miRNA founded in zebrafish.1

It was found that miR-152 was underexpressed in human osteosarcoma (OS) tissues and cell lines. Decreased miR-152 was inversely correlated with lymph-node metastasis and advanced clinical stage. Overexpression of miR-152 significantly inhibited cell proliferation, colony formation, migration and invasion of OS cells. Bioinformatics analyses showed that miR-152 directly targeted E2F transcription factor 3 (E2F3), as further confirmed by a dual-luciferase reporter assay. Dre-miR-152 may be a therapeutic target for OS.1

Moreover, MiR-152 regulated cell proliferation and apoptosis of glioma mediated by Runx2, while the mechanism of down regulated miR-152 in glioma tissues and cells was its hypermethylation.1

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Experimental Validation

This part is sequenced as correct after construction.