Difference between revisions of "Part:BBa K2440029"
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MiRNA locker assembled by using this modularized DNA part was able to bind miR-458 in an Ago2 dependent manner, that is, knockdown of miR-458 was achieved by transfecting cells with miRNA locker. | MiRNA locker assembled by using this modularized DNA part was able to bind miR-458 in an Ago2 dependent manner, that is, knockdown of miR-458 was achieved by transfecting cells with miRNA locker. | ||
| − | Dre-miR-458-5p is a novel RNA founded in zebrafish. And its function is still unknown. | + | Dre-miR-458-5p is a novel RNA founded in zebrafish<sup>1</sup>. And its function is still unknown. |
===Sequence and Features=== | ===Sequence and Features=== | ||
Revision as of 01:15, 1 November 2017
miR-458 target sequence
It is the target sequence of miR-458, a modularized DNA part from a set of chemically synthetic oligo DNA library.
Usage and Biology
MiRNA locker assembled by using this modularized DNA part was able to bind miR-458 in an Ago2 dependent manner, that is, knockdown of miR-458 was achieved by transfecting cells with miRNA locker.
Dre-miR-458-5p is a novel RNA founded in zebrafish1. And its function is still unknown.
Sequence and Features
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Experimental Validation
This part is sequenced as correct after construction.