Difference between revisions of "Part:BBa K2440019"

Revision as of 00:59, 1 November 2017

miR-2467 target sequence

It is the target sequence of miR-2467, a modularized DNA part from a set of chemically synthetic oligo DNA library.

Usage and Biology

MiRNA locker assembled by using this modularized DNA part was able to bind miR-2467 in an Ago2 dependent manner, that is, knockdown of miR-2467 was achieved by transfecting cells with miRNA locker.

Hsa-miR-2467-5p was firstly found in human breast tissue. It may be applied in tumor identity with other miRNAs.¹

By using ChIP-Seq, Genechip and miRNA-Seq techniques, hsa-miR-2467-5p was identified as a NF-κB target miRNA in TNFα-stimulated HeLa Cells.²

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Experimental Validation

This part is sequenced as correct after construction.

@@ Line 9: / Line 9: @@
 MiRNA locker assembled by using this modularized DNA part was able to bind miR-2467 in an Ago2 dependent manner, that is, knockdown of miR-2467 was achieved by transfecting cells with miRNA locker.
-Hsa-miR-2467-5p was firstly found in human breast tissue. It may be applied in tumor identity with other miRNAs.1
+Hsa-miR-2467-5p was firstly found in human breast tissue. It may be applied in tumor identity with other miRNAs.<sup>1</sup>
-By using ChIP-Seq, Genechip and miRNA-Seq techniques, hsa-miR-2467-5p was identified as a NF-κB target miRNA in TNFα-stimulated HeLa Cells2.
+By using ChIP-Seq, Genechip and miRNA-Seq techniques, hsa-miR-2467-5p was identified as a NF-κB target miRNA in TNFα-stimulated HeLa Cells.<sup>2</sup>
 ===Sequence and Features===